Transcription profiling of mouse liver samples comparing hormone sensitive lipase (HSL) knockout mice vs. wildtype

HSL is a key enzyme in in the mobilization of fatty acids from the triglyceride stores of white adipose tissue. In addition, it is expressed in mice liver. In the present microarray study, changes in the transcript profile of murine liver samples due to global HSL knockout were investigated. Experiment Overall Design: Genetic modification to analyze the impact of a general knockout of the HSL gene on liver metabolism. Experiment Overall Design: HSL knockout (ko) mice versus wildtype (wt) mice. Mice fed a normal diet (ND) versus mice fed a high fat diet (FD) for 6 months. Equal amounts of liver samples from six mice were pooled and total RNA was extracted, except for HSL-null mice on FD were only 4 livers were pooled.激素敏感性脂肪酶（Hormone-sensitive lipase，HSL）是白色脂肪组织甘油三酯储存库中脂肪酸动员过程的关键酶。此外，该酶在小鼠肝脏中亦有表达。在本项微阵列（microarray）研究中，我们探究了全身性HSL敲除对小鼠肝脏样本转录谱的影响。实验整体设计：通过基因修饰手段分析HSL基因全身性敲除对肝脏代谢的调控作用；实验分组设置为：HSL敲除（knockout，以下简称ko）小鼠与野生型（wildtype，以下简称wt）小鼠，同时设置正常饮食（normal diet，以下简称ND）组与高脂饮食（high fat diet，以下简称FD）组，两组小鼠均接受为期6个月的饮食干预。唯有高脂饮食组的HSL敲除小鼠仅混合了4份肝脏样本，其余各组均取6只小鼠的等量肝脏样本混合后提取总RNA。