Utility of optimised assay with a lower limit of detection of ≥ 0.004. For a target VAF range the optimal read depth and DNA quantity is indicated to achieve 100% accuracy. Indicated relative total cost and time include raw data file size for 144 samples and the cost for data storage, cost and time for library preparation and quantification, sequencing, and bioinformatic analysis (refer to S7–S8 Tables). Also included are the number (mean and range) of somatic variants observed in community dwelling individuals, and the coefficient of variance (CV) in reference standard and community dwellers at each VAF range.

Utility of optimised assay with a lower limit of detection of ≥ 0.004. For a target VAF range the optimal read depth and DNA quantity is indicated to achieve 100% accuracy. Indicated relative total cost and time include raw data file size for 144 samples and the cost for data storage, cost and time for library preparation and quantification, sequencing, and bioinformatic analysis (refer to S7–S8 Tables). Also included are the number (mean and range) of somatic variants observed in community dwelling individuals, and the coefficient of variance (CV) in reference standard and community dwellers at each VAF range.