Individual high-quality reads (dd-RADsequencing) from: Genomic signatures of clonality in the deep water kelp Laminaria rodriguezii

Individual reads available in the repository come from two double-digest RAD-sequencing libraries (ddRAD-seq), achieved by multiplexing 49 individuals of Laminaria rodriguezii and 130 individuals of Laminaria digitata, including 2 and 5 technical replicates, respectively (see Sample_information.txt). Libraries were prepared according to Peterson et al., (2012) with the following restriction enzymes: PstI and HhaI and then sequenced with 150 bp paired-end reads on Illumina Hiseq 4000 (Génome Québec Innovation core, McGill Univ., Montréal, Canada). The remaining reads (in the directory) were checked for quality using FastQC v.0.11.7, trimmed to 137 bp after the removal of adapters sequencing by Trimmomatic, then demultiplexed according to their barcodes using Stacks's process_radtags (Catchen et al., 2011). Each individual is represented by forward and reverse read-pair (e.g. "Name".trim.R1.fq.gz and "Name".trim.R2.fq.gz). Individual high-quality reads of Laminaria digitata are avaible at: http://www.ncbi.nlm.nih.gov/bioproject/720920.