MoTrPAC - Endurance Exercise Training Study In 6 Months Old Rats [RRBS]

The goal of the endurance exercise training study in young adult rats was to perform exercise training studies in young adult (6-month-old) F344 rats, and from these rats collect multiple tissues in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia. For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max and training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.本研究针对年轻成年大鼠开展耐力运动训练实验，旨在对6月龄的F344大鼠实施运动训练，并采集多组织样本，为化学分析实验室开展精细化检测提供高质量实验材料。 久坐对照组大鼠（10~12只）与8周训练组同步采样。8周训练组与对照组来自同一队列，处死后年龄一致。对于年长组大鼠，额外设置一组对照组（样本量n=5~6），与1~2周训练组同步采样。 大鼠分为久坐对照组与运动训练组。运动训练组大鼠每周5天在啮齿类动物跑台上进行运动，采用渐进式训练方案，运动强度设定为约最大摄氧量（VO2max）的70%，训练时段安排在每日10:00至17:00之间，每周连续训练5天。 训练初始阶段，跑台设置为最大摄氧量70%的强度、坡度5度，单次运动时长20分钟。此后每日增加1分钟运动时长，直至训练第31天（第7周初期），此时单次运动时长达到50分钟。受跑台参数限制，每次训练的速度与坡度会以更大幅度提升。 训练的最高强度与时长自第31天起确立，并在实验方案的最后10天维持该强度，以确保达到稳态。 若大鼠无法完成每周至少4天的训练，则将其剔除实验并处死。 需特别说明的是，跑台初始速度因大鼠的性别与年龄而异。初始速度与最大速度均基于通过预训练检测的合规大鼠的最大摄氧量结果确定。 分配至对照组的大鼠作息安排与训练组相似：每周5天，每日将其置于跑台一侧跑道15分钟，跑台设置为0米/分钟的速度，坡度与训练组当日使用的坡度一致。