RNA-seq analysis of Fgsrp2 and Fgsrp1srp2 mutants

Vegetative hyphae of PH-1, Fgsrp2 and Fgsrp1srp2 mutants were harvested from 16-hour YEPD cultures. Total RNA samples were extracted with the Qiagen RNeasy Micro kit and poly(A)+ mRNA was enriched with immobilized oligo (dT) as described (Liu, et al. 2017). Two biological replicates were prepared for each strain. Strand-specific RNA-seq libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit and sequenced with Illumina HiSeq-2500 with a 2x150 bp paired-end read mode at the Novogene Bioinformatics Institute (Beijing, China).