Investigating the noncanonical Nfkb2 pathway regulation of gene expression in modulating dendritic cell function.

Dendritic cells (DCs) orchestrate intestinal inflammation in health and diseases. We found that human IBD was associated with heightened non-canonical NF-?B signaling in intestinal DCs. The non-canonical NF-?B pathway, which induces RelB:p52-mediated immune gene expressions, has been implicated in DC functions and that genetic inactivation of RelB:p52 in DCs alleviated experimental colitis in mice. Here, we aim to investigate the regulation of gene expression by noncanonical Nfkb2 pathway in modulating DC function. Overall design: The dendritic cell were cultured from the bone marrow of C57BL/6 WT mice and the Nfkb2-/- mice using a GMCSF and IL4 cocktail. The total RNA was isolated from these BMDCs and subjected to RNASeq analysis.