Western Blot analysis of WT and LSD1-GT mES cells.
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https://data.mendeley.com/datasets/d9876prwnx
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资源简介：
Western Blots were conducted whole cell lysates on WT mES and LSD1-GT mES cells with antibodies against various histone modifications and LSD1 (KDM1A). Cells were lysed with 2X Laemmli Buffer and were loaded at various concentrations on 12% acrylamide gels and probed with the following antibodies.
anti-H3K4me1 (ab8895, Abcam)
anti-H3K4me2 (ab7766, Abcam)
anti-H3K4me3 (ab8580, Abcam)
anti-H3K27ac (39135, Active Motif), and
anti-LSD1 (ab17721, Abcam)
For all images,
Lane 1: WT mES 3ul
Lane 2: WT mES 6ul
Lane 3: WT mES 12ul
Lane 4: LSD1-GT mES 3ul
Lane 5: LSD1-GT mES 6ul
Lane 6: LSD1-GT mES 12ul
Protein ladder was loaded on the lane left to Lane 1
创建时间：
2017-02-18
