dsRNA formation during growth and development in yeast

Yeast expresses hundreds of transcripts annotated as long noncoding RNAs during mitosis and meiosis including numerous antisense transcripts that overlap sense mRNAS. We sought to identify sense/antisense pairs that form dsRNAs in vivo in dividing and differentiating yeast cells.酵母在有丝分裂与减数分裂过程中可表达数百条被注释为长链非编码RNA（long noncoding RNA）的转录本，其中包含大量与有义信使RNA重叠的反义转录本。本研究旨在鉴定在分裂与分化的酵母细胞中体内形成双链RNA（double-stranded RNA，dsRNA）的有义/反义转录本对。