Generation Sequencing Facilitates Quantitative Analysis of Wild Type and AGC1-4 Transcriptomes

To further study the molecular mechanism of AGC1-4 in regulating the seed size and to search the genes that the transcription levels have been infected by AGC1-4 gene, we selected the siliques at the globular embryo stage at 3 days after pollination (DAP) from agc1-4 homozygous mutant and the wild-type separately and proceeded the RNA sequencing. Three biological replicates experiments were proceeded and six samples were obtained and a total of 137,259,154 reads were generated. A differentially expressed genes statistic was made under a standard of 1.5 fold change and P-value < 0.05. Compared with wild-type, 1611 differentially expressed genes (DEGs) were identified, including 996 up-regulated genes and 615 down-regulated genes. Overall design: The siliques at 3 days after pollination (DAP) from agc1-4 mutant and the wild-type were generated by the RNA sequencing, in triplicate, using the Illumina HiSeqTM 2500