metagenomic next-generation sequencing of plasma improves the clinical coincidence rate of pathogen diagnosis in patients with suspected sepsis. undefined

Metagenomic second-generation sequencing (mNGS) can detect all pathogenic microorganisms without bias, which is of great significance for the early diagnosis of sepsis. The purpose of this study was to analyze the clinical coincidence rate of mNGS test results of blood samples. In this study, 99 samples of suspected blood flow infection were included for plasma mNGS, and the correlation between mNGS results and blood culture results, serum inflammatory indexes and clinical symptoms was analyzed. The results showed that the detection sensitivity of mNGS test was 83.02%, while that of blood culture was 35.85%, while the clinical coincidence rates of mNGS test and blood culture were 58.49% and 20.75% respectively. Because 32% of the patients with sepsis in this study were secondary to pulmonary infection, compared with alveolar lavage fluid, the detection rate of plasma mNGS was 62.5%. At the same time, the sampling was convenient, noninvasive and easier for clinical application. This study shows that in patients with suspected blood flow infection, the clinical coincidence rate of plasma mNGS detection technology is higher, which is more conducive to early clinical diagnosis and treatment.