Refolding chromatography with immobilized mini-chaperones
收藏PubMed Central1997-04-15 更新2026-04-25 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC20481/
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资源简介：
Mini-chaperones (e.g., a peptide consisting of residues 191-345 of GroEL) that are immobilized on agarose have very efficient chaperoning activity with several proteins that are otherwise recalcitrant to renaturation by conventional methods. We have used immobilized mini-chaperones both in column chromatography and batchwise to renature an insoluble protein from an inclusion body, to refold apparently irreversibly denatured proteins, and to recondition enzymes that have lost activity on storage. Refolding chromatography offers an efficient and simple means to renature proteins in high yield and with biological activity.
提供机构：
National Academy of Sciences
创建时间：
1997-04-15
