Angiotensin Converting Enzyme (ACE) expression in microglia reduces amyloid β deposition and neurodegeneration by increasing SYK signaling and endolysosomal trafficking.

Angiotensin Converting Enzyme (ACE) has been studied for its role in the regulation of myeloid cell bactericidal and antitumor activity. Here, we generated a mouse model with Tamoxifen-dependent ACE expression with Cx3CR1-Cre control. These mice were crossed to 5xFAD and 9 month forebrains were harvested for their nuclei to assess the ACE-dependent changes in amyloid-reactive microglia. Dissociated nuclei were stained with NeuN, Sox9 and Sox10 and microglia were enriched by negatively selecting for these markers.