WhiB1 transcriptone in response to NO stress

Mycobacterium tuberculosis is the etiological agent of tuberculosis. Mtb is an efficient pathogen partially due to its ability to adapt to the disparate conditions encountered during the course of infection. NO is one of the potent antimicrobial chemicals produced in hosts to tackle pathogens. Mtb is known to respond swiftly to NO-stress. Since, WhiB1 is [4Fe-4S] cluster containing transcription factor with high sensitivity to NO, we evaluate the role of WhiB1 in regulating the cellular transcriptional profile of Mtb in presence or absence of NO. Overall design: This study involves determining the mRNA transcriptome profiles for WT and whiB1 knockdown; grown under standard in vitro conditions (7H9+ADS+Tween80 at 37°C and 180 rpm) in presence or absence of NO (DETA-NO). Knockdown was obtained by addition of 200 ng/ml anhydrotetracycline (ATc). Two independent experiments were done for each of the strains.