Myeloid cell analysis during a pro-fibrotic ischemia-reperfusion and cardiotoxin muscle injury.

Myeloid cells are critical to the development of fibrosis following muscle injury, however, the mechanism of their role in fibrosis formation remains unclear. Here we demonstrate that myeloid cell-derived TGF-β1 signaling is increased in a pro-fibrotic ischemia-reperfusion and cardiotoxin (IR/CTX) muscle injury model. This study analyzes scRNAsequencing from the fibrotic region after a pro-fibrotic ischemia-reperfusion and cardiotoxin muscle injury. We used 10X genomics to perform single cell sequencing of cells from the fibrotic area after a pro-fibrotic ischemia-reperfusion and cardiotoxin muscle injury.