Intestinal and thymic tuft cell RNA-seq

The goal of the study was to sequence mRNA from tuft cells (identified as CD45-;EpCAM+;IL-25+ using Flare25 reporter mice) in the epithelia of thymus and small intestine. As a control, non-tuft epithelial cells (CD45-;EpCAM+;IL-25-) were also isolated. The data were used to compare tuft cell markers between the small intestine and thymus. Four replicates of tuft cells were sorted from both tissues. As a reference, four replicates of non-tuft cells were also sorted from the small intestine. One tuft replicate from the small intestine was discarded because it failed QC. One non-tuft replicate was also discarded because of failed QC