RNA-seq analysis of gene expression profile in Setd2 WT and KO germinal center B cells and Phf19 WT and KO germinal center B cells. RNA-seq analysis of gene expression profile in Setd2 WT and KO germinal center B cells and Phf19 WT and KO germinal center B cells

The goals of this study are to compare gene expression profiles of Setd2 WT and KO germinal center B cells and reveal the underlying mechanisms by which Setd2 regulates germinal center B cell responses. Further our study aims to compare gene expression profiles of Phf19 WT and KO germinal center B cells reveal the underlying mechanisms by which Phf19 regulates germinal center B cells responses. Finally, we combine the two profiles and investigate the relation shape betweeen Setd2 dependent regulation and Phf19 dependent regulation. Overall design: The germinal center B cell specific Setd2-deficient mice and WT control mice were immunized and 10e6 germinal center B cells were FACS sorted for RNA extraction, and the germinal center B cell specific Phf19-deficient mice and WT control mice were immunized and 10e6 germinal center B cells were FACS sorted for RNA extraction. Library were then generated with illumina protocol and subject to NGS and bioinformatic analysis.