CRISPR/Cas9-mediated fine-tuning of miRNA expression in tetraploid potato

Three CRISPR-edited MIR160a (cr-MIR160a) and MIR390a (cr-MIR390a) transgenic lines of potato were sequenced using an Illumina NovaSeq system (50 bp single-end at 20 milion read depth). sRNA sequencing approach has been used to experimentally confirmed whether CRISPR-Cas9 induced mutations in transgenic lines affected pre-miRNA precursor processing or instead provide Dicer-like proteins with a new template to generate new miRNA variants from the mutated pre-miRNA precursors.