Population genetic structure of the gastropod species Bulinus truncatus

Background: Gastropod snails remain strongly understudied, despite their important role in transmitting parasitic diseases. Knowledge on their distribution and population dynamics increases our understanding of processes driving disease transmission. This is the first study using High Throughput Sequencing (HTS) to elucidate the population genetic structure of the hermaphroditic snail Bulinus truncatus (Gastropoda, Heterobranchia) on a regional (17 to 150 km) and an inter-regional (1,000 – 5,400 km) scale. This snail species acts as an intermediate host of Schistosoma haematobium and Schistosoma bovis, which cause human and animal schistosomiasis respectively. Methods: Bulinus truncatus snails were collected in Senegal, Cameroon, Egypt and France and identified through DNA barcoding. A single-end Genotyping by Sequencing (GBS) library, comprising of 87 snail specimens from the respective countries, was built and sequenced on an Illumina HiSeq 2000 platform. Reads were mapped against S....研究背景：腹足类软体动物在寄生虫病传播中发挥着关键作用，但目前相关研究仍严重不足。对该类群分布与种群动态的认知，有助于深化我们对驱动疾病传播的核心过程的理解。本研究首次采用高通量测序（High Throughput Sequencing, HTS）技术，在区域尺度（17~150 km）及跨区域尺度（1000~5400 km）下，解析雌雄同体软体动物截形布氏螺（Bulinus truncatus，腹足纲、异鳃亚纲）的种群遗传结构。该螺类分别是引发人体血吸虫病的埃及血吸虫（Schistosoma haematobium）与引发动物血吸虫病的牛血吸虫（Schistosoma bovis）的中间宿主。 研究方法：研究人员在塞内加尔、喀麦隆、埃及及法国采集截形布氏螺样本，并通过DNA条形码（DNA barcoding）完成物种鉴定。构建了包含来自上述四国的87份螺类标本的单端测序分型（Genotyping by Sequencing, GBS）文库，并在Illumina HiSeq 2000测序平台上完成测序。测序reads被比对至S....