Gene expression in migtating TAMs

We have employed whole genome microarray expression profiling as a discovery platform to identify different gene expression in normal,tumor-primed or tumor-primed migrating macrophages. Peritoneal macrophages were cultured alone as control group (Ctrl) or co-cultured with CT-26 cells (Group T) in the lower chamber of Transwell. The migratory macrophages co-cultured with CT-26 cells from upper chamber of the Transwell were marked as group M. Those macrophages were then isolated and subjected to microarray assays. Each group contain 3 samples.