Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability

Degradation of most yeast mRNAs involves decapping by Dcp1/Dcp2. DEAD-box protein Dhh1 has been implicated as an activator of decapping, and as a translational repressor, but their functions in cells are incompletely understood. We have analyzed these questions by a combination of ribosome profiling, RNA-Seq, CAGE analysis of capped mRNAs.大多数酵母信使RNA（mRNA）的降解过程依赖Dcp1/Dcp2介导的脱帽反应。DEAD盒蛋白（DEAD-box protein）Dhh1被证实可作为脱帽反应的激活因子，同时兼具翻译抑制功能，但其在细胞内的具体生物学功能仍未被完全阐明。本研究结合核糖体谱分析（ribosome profiling）、RNA测序（RNA-Seq）以及带帽mRNA的CAGE（Cap Analysis of Gene Expression）分析，对上述相关问题展开了系统性探究。