The rice anther transcriptomes responded to low temperature

Transcriptome analysis using a microarray equipped with probes for genes and repetitive sequences has been performed to examine the expression change in rice anther. We performed transcriptome analysis in 13 rice lines with different cold tolerances at the booting stage because the low temperature at this stage resulted in pollen sterility. From our study, it was found that genome-wide expression is negatively correlated with the degree of cold tolerance. The 13 rice lines exposed to the low temperature at 12℃ for four days, were used for the microarray analyses. RNA was extracted from the anthers after the cold-treated and non-treated anthers with two replicates.