RNA sequencing analysis of transcriptomics to study the growth of Lactobacillus salivarius Ren in raffinose. RNA sequencing analysis of transcriptomics to study the growth of Lactobacillus salivarius Ren in raffinose

Purpose: To understand the metabolic mechanism of Lactobacillus salivarius Ren in raffinose Methods: Samples of Lactobacillus salivarius Ren grown in glucose and raffinose were sequenced on the Illumina Hiseq platform. Three independent biological replicates were generated, including a total of six samples. Results: Raw data were firstly processed through in-house perl scripts to generate clean data, and then clean date were mapped to the reference genome, getting about 8-10 million total mapped reads per sample. Overall design: Lactobacillus salivarius Ren is cultured in MRS medium with carbon source as glucose and raffinose