Mus musculus musculus Transcriptome or Gene expression

Retinal ganglion cells were manually isolated based upon their physiological distinction through cell-attached recordings. Single cell RNA-Sequencing was employed for the characterization of transcriptomes to identify gene candidates that were expressed solely in ganglion cell subtypes. Design: Retinas from C57BL/6 mice were dissected under IR light (940 nm), various visual stimuli were projected upon the retina and the response of each RGC was monitored. Once a given cell was classified, a fire-polished glass electrode was used to aspirate the soma and transfer it to a nuclease-free PCR tube for processing. Single cell libraries were generated using SMART-seq protocols. The resulting samples were pooled and sequenced on an Illumina 2500, with an average read length of 100bp at the Iowa State University DNA Facility.