Data from: Optimising glyphosate tolerance in rapeseed (Brassica napus L.) by CRISPR/Cas9-based geminiviral donor DNA replicon system with Csy4-based single-guide RNA processing

The files were the vector sequence and annotationin used in the Journal article "Optimising glyphosate tolerance in rapeseed (<em>Brassica napus</em> L.) by CRISPR/Cas9-based geminiviral donor DNA replicon system with Csy4-based single-guide RNA processing" (Manuscript Number: JEXBOT/2020/300772), which will publish on the Journal of Experimental Botany. The sequence of original vectors (pTRANS_221, pMOD_A0501, pMOD_B2103, pMOD_C2515, and pKSE401) was downloaded from Addgene. We cloned the <em>BnC04EPSPS </em>(BnC04g56880D)gene from the rapeseed cultivar 627R, and used overlapping PCR to make <em>BnC04EPSPS </em> Donor templates containing TIPS or LFGAAGMCRL mutations. Finally, SnapGene 3.2.1 were used to annotate all the modified vectors. Nine vectors were included in this dataset.