Lysine fatty-acyl H3 peptide pulldown with HeLa SILAC nuclear extract

Histone H3 peptides with acyl modification on either K9 or K27 were used for peptide pull-down with stable isotope (light: K0R0, heavy: K8R10) labeled Hela nuclear extract. The acyl modifications are: acetyl (Ac), propionyl (Pro), Butanoyl (But), crotonyl (Cr), 3-hydroxy butanoyl (bHB), succinyl (Su), Pentanoyl (Vl), Hexanoyl (Xho) and Myristoyl (Myr). Each modified peptide is compared with an unmodified peptide with the same amino acid sequence. Both forward (unmodified-light, acyl-heavy) and reverse (unmodified-heavy, acyl-light) labeling and pull-down experiments were performed.