Impact of α-arrestin deletion on the abundance of PM proteins affected by the CHX treatment.

Fold-change ratios of the abundance of PM proteins identified in Table 1 between the CHX-treated artΔ and WT strains. The first two columns indicate the UniProtKB accession number and name of proteins. The remaining columns correspond to artΔ+CHX/WT ctrl ratio, specifying the protein relative abundance in the CHX-treated arrestin-knockout strains divided by its relative abundance in the CHX-treated WT. The same total amount of proteins was analyzed in all conditions tested. The red and blue colors specify whether the ratio is significantly higher or lower than 1 after hypothesis testing (i.e., with a p-value artΔ+CHX/WT+CHX > 1) is more abundant in the artΔ+CHX than in the WT+CHX condition. We interpret an increase in surface protein upon α-arrestin deletion (i.e., indicated in red) as reflecting a defect in internalization of that PM protein. Inversely, a protein with a blue ratio (artΔ+CHX/WT+CHX artΔ+CHX than in the WT+CHX. White ratios are not significantly different from one upon hypothesis testing, regardless of their absolute values. Proteins are classified as in Table 1. Displayed ratios correspond to the geometric means from 3 independent experiments.