Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and cd36-/- murine peritoneal macrophage Transcriptomes

Oxidized LDL induce macrophages to turn into foam cells in a CD36 dependent manner. The Goal of this study is to compare transcriptome profile by RNA-seq among WT/CD36 knockout macrophages treated with or without oxLDL. We detected re-organization of lipid metabolisms as well as immune activation by oxLDL. Overall design: Peritoneal macrophages were isolated from WT or CD36 knockout mice and cultured in vitro. Cells then treated with 20µg/ml oxLDL or media (control) for 8h. We triplicate each condition (i.e. WT-control, WT-oxLDL, CD36 KO-control, CD36 KO-oxLDL).