Genome-wide RNA sequencing on myeloid derived suppressor cells from mice treated with lymphodepleting chemotherapy reveals distinct IL-6 driven regulatory patterns

Myeloid derived suppressor cells (MDSCs) were sorted from B16 tumor-bearing mice, WT and IL-6KO, that were untreated or given lymphodepleting cyclophosphamide and fludarbine. We report that monocytic and polymorphonuclear MDSC subsets (M-MDSCs and PMN-MDSCs respectively) have a unique gene expression profile in comparison to MDSCs collected from untreated tumor-bearing animals. Overall design: M-MDSCs and PMN-MDSCs were sorted from WT untreated mice (n=3) and lymphodepleted (LD) mice (n=3); IL-6KO untreated mice (n=3) and lymphodepleted (LD) mice. Lymphodepleting treatment was initated in mice with established tumors and MDSCs were collected from the spleens of mice 7 days after treatment.