Crystal structure of the E192N mutant of E. Coli N-acetylneuraminic acid lyase in complex with pyruvate and the inhibitor (2R,3R)-2,3,4- trihydroxy-N,N-dipropylbutanamide in space group P21 crystal form I
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Crystal structure of the E192N mutant of E. Coli N-acetylneuraminic acid lyase in complex with pyruvate and the inhibitor (2R,3R)-2,3,4- trihydroxy-N,N-dipropylbutanamide in space group P21 crystal form I Descriptor: (2R,3R)-2,3,4-TRIHYDROXY-N,N-DIPROPYLBUTANAMIDE, N-ACETYLNEURAMINATE LYASE Authors: Campeotto, I, Bolt, A.H, Harman, T.A, Trinh, C.H, Dennis, C.A, Phillips, S.E.V, Pearson, A.R, Nelson, A, Berry, A. Deposit date: 2009-08-03 Release date: 2010-08-25 Last modified: 2023-12-20 Method: X-RAY DIFFRACTION (2.05 Å) Cite: Structural Insights Into Substrate Specificity in Variants of N-Acetylneuraminic Acid Lyase Produced by Directed Evolution. J.Mol.Biol., 404, 2010
大肠杆菌(E. Coli)N-乙酰神经氨酸裂解酶(N-acetylneuraminic acid lyase)E192N突变体的晶体结构,该蛋白与丙酮酸盐及抑制剂(2R,3R)-2,3,4-三羟基-N,N-二丙基丁酰胺形成复合物,晶体空间群为P2₁,晶型为I。
描述项:(2R,3R)-2,3,4-三羟基-N,N-二丙基丁酰胺、N-乙酰神经氨酸裂解酶
作者:Campeotto, I、Bolt, A.H、Harman, T.A、Trinh, C.H、Dennis, C.A、Phillips, S.E.V、Pearson, A.R、Nelson, A、Berry, A
提交日期:2009年8月3日
发布日期:2010年8月25日
最后修改日期:2023年12月20日
实验方法:X射线衍射(X-RAY DIFFRACTION),分辨率2.05埃(Å)
引用文献:《定向进化获得的N-乙酰神经氨酸裂解酶变体的底物特异性结构解析》(Structural Insights Into Substrate Specificity in Variants of N-Acetylneuraminic Acid Lyase Produced by Directed Evolution),J.Mol.Biol., 404, 2010
创建时间:
2009-08-03



