Ovarian cancer cells co-cultured with MSC1 differ from MSC2 co-cultures in their secretion of bioactive factors.

SKOV3 ovarian cancer cells were plated on 24-well plates until they reached 50–70% confluence. MSC1, MSC2, (25,000 cells/insert) or medium control were then added into 0.4 µM (no cell-cell contact) or 8 µM transwell inserts and the co-cultures were allowed another 72 hr prior to collecting the conditioned medium and testing by Bio-Plex Cytokine Assays following the manufacturer’s instructions (Human Group I & II; Bio-Rad, Hercules, CA). Arrows represent relative normalized changes compared with the SKOV3 alone control. Biofactor levels that were different between the MSCs grown in 0.4 µM (no cell-cell contact) versus 8 µM transwell inserts are represented by “+.” Those biofactor levels that were similar in both sample groups are represented by “−.” Data are representative of triplicate measurements with 4 MSC donors in at least 4 independent experiments.