Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 05/12/2003 to 11/12/2003 (Hobart - Dumont d'Urville)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 05/12/2003 to 11/12/2003on a trip from Hobart to Dumont d'Urville.Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集自星盘号（RV L'Astrolabe，平台代码：FHZI）科考船，航次时间为2003年12月5日至2003年12月11日，航线从霍巴特前往迪蒙·迪尔维尔站。
光合系统II（Photosystem II, PSII）的最大光化学效率，亦称PSII最大量子产额（Fv/Fm），是浮游植物研究领域应用最为广泛的荧光参数之一。该参数代表潜在光化学效率，即光合装置捕获的光能用于光化学反应的概率。研究表明，Fv/Fm对物理化学性质的变化具有瞬时响应，可作为浮游植物整体健康状态或生存能力的诊断指标。结合PSII吸收截面面积与叶绿素浓度，该参数可用于初级生产力测定（Cheah等，2011，《深海研究》（Deep Sea Research））。
本研究的海水样本取自3米深度，通过船舶洁净海水管路持续输送。
以暗适应状态（暗适应时长不少于15分钟）下的海水为研究对象，采用由100个亚饱和微闪（闪光时长1.1μs，闪光间隔2.8μs）序列与20个弛豫微闪（闪光时长1.1μs，闪光间隔51.6μs）序列组成的闪光组，以1分钟间隔连续测定快速重复率（FRR）荧光产额。