Analysis of IL-17-, IFNγ- or IL-4/5-producing CD4⁺ T cells in the draining LNs after intranasal allergen challenge.

Groups of C3aR−/− and wild-type mice (n = 4–5 per group) were intranasally administered with allergen every other days for four times. One day after the last challenge, lymphoid cells from the lung or draining LNs were obtained, and the expression of IL-17, IFN-γ, and IL-4/5 by CD4+ T cells was analyzed by intracellular staining. Data shown are mean ± SE after gated on CD4 expression, and represent two independent experiments. *, p<0.05 or **, p<0.01 in comparison with wild-type.