Differential gene expression profiling of conventional (Tconv), regulatory (Treg), follicular (TFH) helper and follicular regulatory (TFR) T-cells from control vs OX40L-Jagged-1 treated NZBWF1/j mice

Differential gene expression profiling of conventional (Tconv), regulatory (Treg), follicular (TFH) helper and follicular regulatory (TFR) T-cells from control vs OX40L-Jagged-1 treated NZBWF1/j mice Tconv, Treg, TFH and TFR subsets (n=3) from control vs OX40L-JAG1 treated mice were compared for differential gene expression pattern. 23-week-old female NZBWF1/j mice were injected (i.p.) with recombinant mouse OX40L (2mg/kg body weight) and mouse JAG1 (2mg/kg body weight) once a week for three consecutive weeks. Age and sex-matched control mice received PBS. On week 26, mice were sacrificed and Tconv (CD4+CXCR5-GITR-) cells, Tregs (CD4+CXCR5-GITR+), TFH (CD4+CXCR5+PD1Hi GITR-/low), and TFR (CD4+CXCR5+PD1Hi GITRHi) cells were sorted and subjected to RNA-seq analysis.