Changes in Microbial Diversity Over Tidal Cycles (Analog Site, Dauphin Island Beach)

This dataset provides information about spatial and temporal heterogeneity of environmental gradients (e.g. bacterial community composition of sediment and water, sediment properties, water quality, and atmospheric physicochemistry) on an exposed sandy beach intensively sampled over diurnal tidal cycles. Twenty parallel intertidal beach transects, perpendicular to the shoreline, were marked and excavated over five days. Field measurements and observations include water quality (temperature, pH, conductivity, dissolved oxygen concentration) recorded at each sampling time using meters; atmospheric physicochemistry (solar radiation, temperature, wind speed, barometric pressure, etc.) at each sampling time and transect location; tide height, water level and wave frequency; and other observations made at the time of collection of sediment and water sample collection. Filtered water samples were used for DNA isolation. Bacterial community composition for beach surface sediment and water are provided at the phylum and Proteobacteria class levels for four of the twenty transects (Day 1).本数据集记录了一处裸露潮间带沙滩在全日潮汐周期内开展密集采样所得到的环境梯度时空异质性相关信息，具体涵盖沉积物与水体的细菌群落组成、沉积物理化特性、水质以及大气理化性质等。研究期间共布设20条相互平行的潮间带沙滩断面，所有断面均与海岸线垂直，并在5天内完成标记与开挖采样作业。野外实测与观测内容包括：各采样时刻通过便携仪器记录的水质指标（水温、pH值、电导率、溶解氧浓度）；各采样时刻及对应断面位置的大气理化参数（太阳辐射、气温、风速、大气压力等）；潮汐高度、水位与波浪频率；以及沉积物与水体样本采集时同步开展的其他观测记录。经过滤处理后的水体样本用于脱氧核糖核酸（DNA）的分离提取。本数据集提供了20条断面中4条断面（第1天）的沙滩表层沉积物与水体的细菌群落组成信息，分类阶元覆盖门水平及变形菌门（Proteobacteria）纲水平。