Twin-miRs and their many weak targets. Drosophila melanogaster

MicroRNAs (miRNA) are small, endogenous RNAs that regulate the expression of mRNAs posttranscriptionally. To identify miRNAs that produce twin products, we sequenced these five small RNA libraries and collected other published datasets to represent a systematic coverage of the major tissue types (head, body and sexual organs) in D. melanogaster. To survey the repression effects of twin miRs on the their targets, we dissected testes from adult flies that lacked Dm310s due to a knock-out mutation (Tang, et al. 2010) and performed an RNA-seq analysis. Overall design: Five small RNA samples mainly from reproductive organs, and four RNA-seq samples from testes, were analyzed in D. melanogaster. Please note that the 'CanS testes' raw data fastq file is unavailable and only fasta file containing clean reads and read counts was generated as following; the sequenced reads were trimmed for adaptor sequence, masked for low-complexity or low-quality sequence, and collapsed to the fasta format, on which the further analysis was carried out. The Gene_Expression_log2(RPKM).txt contains two additional data columns - miR-313-3p_target and miR-313-5p_target - showing whether it is a TargetScan predicted target of dme-miR-313-3p or -5p, respectively. Both 'Dm310s mutant' and 'Control' flies are generated from the stock EP(2)2587 [P(3)l(2)05510EP2587; Szeged Stock Center)]. The detailed construction method is described in Tang, T., et al. (2010; PMID 20615957)