Single-nuclei gene expression profiling of human SMARCB1-deficient peripheral T cell lymphomas

Peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) is a heterogeneous group of malignancies with poor outcome. Here we identified a subgroup, PTCL-NOS SMARCB1-, which is characterized by the lack of SMARCB1 protein expression and is more common in young patients under 25 years of age. To investigate the cellular heterogeneity of PTCL-NOS SMARCB1- and the role of its tumor microenvironment, we performed single-nuclei RNA sequencing (snRNA-seq) of five patient samples. Gene expression profiles of PTCL-NOS SMARCB1- patients (n = 5) were generated by snRNA-seq of formalin-fixed, paraffin-embedded (FFPE) tissue samples using Chromium Fixed RNA Profiling for Multiplexed Samples technology (10X Genomics).