Supplementary Material for: The Association Between Gene Expression Profiling and Regression Rate of Posterior Uveal Melanoma Following Proton Beam Irradiation

Introductions: This study evaluated the association between gene expression profiling (GEP), PRAME (preferentially expressed antigen in melanoma) and regression rate of uveal melanoma after proton beam irradiation (PBI)

Methods: A retrospective review of uveal melanoma patients treated with PBI between 2013 and 2021, with GEP results and at least 3 post-radiation ultrasound measurements, was conducted. Patients with local recurrences were excluded. Regression rates were analyzed using a linear mixed model to predict percentage change in thickness from baseline. Cox regression was conducted to determine whether slow or fast regression, based on the median regression rate at 18 months, correlates with metastasis risk.

Results: The study included 106 patients, with GEP classifications of 1A in 43.4%, 1B in 25.5%, and 2 in 31.1%. Overall, the mean change in tumor thickness was 20.9%, 35.1%, 51.4% and 59.3% at 1 year, 2 years, 4 years, and 6 years, respectively. No differences in regression rates between GEP classes and PRAME expression were found through 72 months post PBI. The median regression at 18 months was 27.1%. Slow and fast regression was not associated with the risk of metastasis.

Conclusion: No association between GEP, PRAME and regression rate was found through 72 months post PBI.

引言：本研究评估了基因表达谱（GEP）、PRAME（黑色素瘤优先表达的抗原）与经质子束照射（PBI）后脉络膜黑色素瘤的退缩率之间的关联。
方法：对2013年至2021年间接受PBI治疗的脉络膜黑色素瘤患者进行回顾性审查，纳入标准包括GEP检测结果及至少3次放疗后的超声测量数据。排除有局部复发的患者。使用线性混合模型分析退缩率，以预测基线厚度百分比的变化。通过Cox回归分析，基于18个月时的中位退缩率，评估缓慢或快速退缩是否与转移风险相关。
结果：研究纳入了106名患者，其中GEP分类为1A占43.4%，1B占25.5%，2型占31.1%。总体而言，肿瘤厚度在1年、2年、4年和6年时的平均变化率分别为20.9%、35.1%、51.4%和59.3%。在PBI后72个月期间，未发现GEP类别与PRAME表达之间的退缩率存在差异。18个月时的中位退缩率为27.1%。缓慢和快速退缩与转移风险无相关性。
结论：在PBI后72个月内，未发现基因表达谱、PRAME与退缩率之间存在关联。