U2OS Cell Cycle Transition Curve-- NCS 100 ng/mL

U2OS cells expressing the PCNA-mCherry reporter were induced with 100 ng/mL NCS at 20.4 hours into the movie and then imaged for another 48 hours. Time-lapse images were taken every 20 minutes.表达PCNA-mCherry报告基因（PCNA-mCherry reporter）的U2OS细胞（U2OS cell），在该延时成像实验进行至第20.4小时时，以100 ng/mL浓度的新制癌菌素（NCS，Neocarzinostatin）进行诱导处理，随后继续成像48小时。本次实验采用延时成像方案，每20分钟采集一帧图像。