Manuscript adx9450 - Source data and codes for snSEQ

This dataset includes single-nucleus RNA sequencing (snRNA-seq) data derived from rabies tracing with nuclear-localized GFP, designed to capture both cell-type-specific and input-specific gene expression for the ESR1+ LHA-LHb pathway. Single-nucleus RNA sequencing (snRNA-seq) was performed on mouse tissue from the Nucleus Accumbens (ACB), the Bed Nuclei of the Stria Terminalis (BNST) and the Lateral preoptic area (LPO). Adult Esr1-cre mice first received unilateral injections of 150 nl of helper virus (AAV-EF1α-DIO-TVA-V5-WRPE-hGHpA) in the LHA. Three weeks after, the same animals received unilateral injections of 200 nl of RV-GFP into the LHb. The mice were euthanized 10 days after the injection of the RV-GFP, and the tissue was collected and processed. This file possess: 

the custom code used for the clustering and subclustering of the single nuclei RNA sequencing data (Clustering_h2b_before_sub.Rmd and Sub_clustering_striatal_clusters_h2b.Rmd)

the raw files used for the analysis ("raw" folder)

the processed data obtained upon clustering and subclustering ("processed" folder)

the plots generated from the analysis of the data ("plots" folder)

For any additional informations, please contact the corresponding authors of the manuscript: [e-mail], [e-mail]