Gene expression in commercial human cerebral microvascular endothelial cells (hCMECs) line knock out or not for RNF213

To study the role of RNF213, we generated RNF213-deficient human cerebral microvascular endothelial cells by the CRISPR-Cas9 double nickase method and showed that RNF213 may be an important regulator of the blood-brain barrier permeability and homeostasis One commercial line of hCMECs at passage 33 cultivated in proliferation or at confluency and transfected or not (wild type) with Cas9 without guide RNA (transfection control) or with ZNF213 specific guide RNA (knock-out ZNF213)