Lipidomics analysis (PC) from water bottle samples in and around a cyclonic eddy during METEOR cruise M156, off Mauritania@en

This dataset includes lipidomics data over the surface mixed layer (0-35 m depth) of eleven stations with five of them inside or in the vicinity of a mesoscale cyclonic eddy that formed off Mauritania along a 900 km zonal corridor between Mauritania and the Cabo Verde islands in the eastern Tropical North Atlantic during the M156 cruise on the RV Meteor from July 3rd to August 1st 2019. Lipid samples were collected onto 0.22 µm Durapore filters (Millipore) and immediately flash-frozen and stored at −80 °C until processing. Extraction was performed using a modified Bligh and Dyer (Bligh and Dyer, 1959; https://doi.org/10.1139/o59-099) method described in Popendorf et al. (2013; https://doi.org/10.1007/s11745-012-3748-0). Lipid extracts were analyzed by HPLC-ESI-MS/MS on a Bruker maXis Ultra-High Resolution quadrupole time-of-flight tandem mass spectrometer (Bruker Daltonik, Bremen, Germany) equipped with an ESI ion source operating in positive mode. The HPLC-ESI-MS method is described in Wörmer et al., 2013 (https://doi.org/10.1016/j.orggeochem.2013.03.004). Lipids were identified by retention time as well as accurate molecular mass and isotope pattern match of proposed sum formulas in full scan mode and MS/MS fragment spectra. Lipid identities are resolved only to the level of lipid species, i.e., the sum of the properties of the substituents at both the sn-1 and sn-2 positions. After identification, quantification of analytes was performed using a series of standard curves, followed by normalization to concentration of an internal standard.本数据集涵盖2019年7月3日至8月1日期间，于“流星号”（RV Meteor）科考船执行M156航次时，在热带北大西洋东部毛里塔尼亚外海、毛里塔尼亚与佛得角群岛之间900公里纬向走廊内生成的中尺度气旋涡及其周边区域11个站位的表层混合层（水深0~35米）脂质组学数据，其中5个站位位于该气旋涡内部或其邻近海域。脂质样品采用0.22 μm Durapore（默克Millipore）滤膜收集，采集后立即进行速冻，并于-80℃条件下保存直至后续处理。脂质提取采用经改良的布莱根-代尔（Bligh and Dyer, 1959; https://doi.org/10.1139/o59-099）法，具体操作参照Popendorf等（2013; https://doi.org/10.1007/s11745-012-3748-0）发表的改良方案。脂质提取物采用高效液相色谱-电喷雾电离串联质谱（HPLC-ESI-MS/MS）进行分析，所用仪器为德国不来梅布鲁克道尔顿（Bruker Daltonik, Bremen, Germany）生产的布鲁克maXis超高分辨四极杆-飞行时间串联质谱仪，配备正离子模式电喷雾电离（ESI）源。该HPLC-ESI-MS分析方法详见Wörmer等（2013; https://doi.org/10.1016/j.orggeochem.2013.03.004）的研究成果。脂质的鉴定通过保留时间、精确分子量，以及全扫描模式下候选分子式的同位素分布匹配结果与MS/MS碎片谱图比对完成。本次研究仅将脂质鉴定至脂质物种（lipid species）级别，即仅明确sn-1与sn-2位取代基的总属性。完成鉴定后，通过一系列标准曲线对分析物进行定量，并以内标物浓度作为参照完成归一化处理。