Transcriptional profiling of drug-induced changes in mice nucleus accumbens and prefrontal cortex

We applied next-generation sequencing to analyse changes in the expression of Dclk1 gene isoforms in the brain in response to several psychoactive drugs with diverse pharmacological mechanisms of action. We used bioinformatics tools to define the range and levels of Dclk1 transcriptional regulation in the mouse nucleus accumbens and prefrontal cortex Overall design: Sequencing was performed on ribo-depleted RNA samples derived from the mouse nucleus accumbens septi (Nac) and prefrontal cortex (PFCx) 2 hours after treatment with antidepressants (venlafaxine and mianserin), antipsychotics (haloperidoland risperidone), a psychostimulant (methamphetamine) or a psychotomimetic (ketamine)