Transcriptional profiling of striatum and cortex from a mouse model of Huntington's disease (Q140) crossed with Mlh1 heterozygous mice [Mlh1]

As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntingtons disease (HD) through various computational modeling efforts. Striatum and cortex were isolated from 6-month-old Mlh1+/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 4 genotypes: WT, Mlh1+/-, Q140, and Q140 X Mlh1+/-, 8 replicates per genotype. RNASeq analysis was performed on samples from the striatum and cortex tissues of 6-month-old Mlh1+/-, Q140 KI, Q140 X Mlh1+/-, and wild-type mice. N = 8 per group (3-4F/4-5M).本数据集为加州大学洛杉矶分校（University of California, Los Angeles, UCLA）与CHDI合作项目的组成部分。UCLA团队针对经各类计算建模研究证实与亨廷顿舞蹈症（Huntington's disease, HD）相关的123个基因构建了基因敲除（knockouts, KOs）模型。研究人员从与Q140敲入（knock-in, KI）HD小鼠及其对应的野生型对照杂交获得的6月龄Mlh1+/-小鼠体内分离纹状体与大脑皮层组织，针对4种基因型的样本开展转录组测序（RNASeq）分析，涉及基因型包括野生型（wild-type, WT）、Mlh1+/-、Q140以及Q140与Mlh1+/-的杂交基因型，每种基因型设置8个生物学重复。本研究的转录组测序样本均取自6月龄Mlh1+/-、Q140敲入型、Q140与Mlh1+/-杂交型以及野生型小鼠的纹状体和皮层组织，每组样本量为8（雌性3-4只，雄性4-5只）。