Vitis vinifera Ovules Transcriptome

Thompson Seedless (TS) (Vitis vinifera L., stenospermocarpic, seedless) and Pinot Noir (PN) (V. vinifera L., seeded) were grown in the Germplasm unit of Northwest Agriculture and Forests University, and managed following standard local fertilization, irrigation and pest-management practices. In 2013 and in 2014, ovules were isolated from TS and PN 20, 30, 40 and 50 days after full flowering (DAF) (full flowering is defined as when more than 75% caps are off), Ovules were immediately stored at -80? pending analysis. Fresh weight, and longitudinal and transverse diameters of ovules and berries were measured using digital calipers. For each experiment, three biological replicates of 30 berries and ovules were used.RNA quality and quantity were first checked by Bioanalyzer 2200 (Agilent) and then stored at -80?. High-throughput RNA sequencing (RNA-seq) was carried out by Novogene using NEBNext: emoji: UltraTM RNA Library Prep Kit, and 100 base-paired end sequencing on the Illumina Hiseq-2000 platform.