DNA-seq of Xylella fastidiosa OMVs and WCL

Xylella fastidiosa is a Gram-negative plant pathogen responsible for severe diseases in a variety of economically important crops. A critical aspect of its pathogenicity is the production of outer membrane vesicles (OMVs). While some knowledge exists on the molecular cargo of X. fastidiosa (Xf)-OMVs, nucleic acid cargoes remain unknown. Since OMVs protect DNA from degradation during transfer, vesiduction increases the efficiency of HGT, aiding bacterial evolution and adaptation. In order to understand if this mechanism is relevant for Xf, we sequenced DNA extracted from OMVs and compared it to DNA extracted from whole bacterial cells.