Additional file 1: of Xylan degradation by the human gut Bacteroides xylanisolvens XB1AT involves two distinct gene clusters that are linked at the transcriptional level

Table S1. RNA-seq mapping assessment. Table S2. Xylanase specific activity of B xylanisolvens XB1AT. Table S3. Proteins identified by MALDI-TOF MS or LC-ESI-MS/MS over-produced upon growth of B. xylanisolvens XB1AT on OSX relative to xylose. Table S4. Composition of the commercial oat-spelt xylan (SERVA, France) used in this study. Table S5. Primers used for RT-PCR (to amplify the intergenic regions between two consecutive ORFs within PUL 43). Table S6. Primers used for relative RT-qPCR. Table S7. Primers used for insertion mutagenesis into PUL 43 HTCS gene (BXY_29350). Figure S1. Growth of B. xylanisolvens XB1AT (Wt) and PUL 43 HTCS (BXY_29350) mutant on glucose, xylose, wheat arabinoxylan (WAX) and oat-spelt xylan (OSX). Figure S2. B. xylanisolvens XB1AT gene expression in response to oat-spelt xylan (OSX) and xylose relative to glucose obtained from RNA-seq analysis. Figure S3. B. xylanisolvens XB1AT PUL expression in response to xylose relative to glucose at late-log phase obtained from RNA-seq analysis. Figure S4. Schematic layout of the mutant construction and validation of pGERM:HTCS insertion into PUL 43 HTCS gene (BXY_29350) of B. xylanisolvens XB1A genome. (XLSX 454Â kb)