Exploring the mRNA and Plasma Protein Levels of BDNF, NT4, SIRT1, HSP27, and HSP70 in Multiple Sclerosis Patients and Healthy Controls

Multiple Sclerosis sclerosis (MS) is a chronic, autoimmune neurodegenerative disease affecting the central nervous system. It is a major cause of non-traumatic neurological disability among young adults in North America and Europe. This study focuses on neuroprotective genes (BDNF, NT4/5, SIRT1, HSP70, and HSP27). Gene expression and protein levels of these markers were compared between MS patients and healthy controls. BThe blood samples were collected from 42 patients with multiple sclerosis (MS) and 48 control subjects without MS. Quantitative real-time PCR was performed to measure the expression of specific genes. The samples were analyzed in duplicate, and the abundance of mRNA was quantified using the 2-ΔCt method. ELISA assay was used to measure the concentration of specific proteins in the plasma samples. Results The results show that a 3.5-fold decreased in the gene expression of BDNF by 3.5-fold corresponds to a 1.5-fold downregulation in the associated plasma protein concentration (P < 0.001). Similar trends were observed with NT-4 (5five-fold decrease, slight elevation in protein), SIRT1 (two2-fold decrease, two2-fold protein decrease), HSP70 (4four-fold increase, nearly 2two-fold protein increase), and HSP27 (four4-fold increase, two2-fold protein increase) (P < 0.001). The This study reveals strong correlations between gene expression and protein concentration in MS patients, emphasizing the relevance of these neuroprotective markers in the disease.

多发性硬化症（MS）是一种慢性、自身免疫性神经退行性疾病，影响中枢神经系统。该疾病在北美和欧洲是导致年轻成人非创伤性神经功能障碍的主要原因。本研究聚焦于神经保护基因（包括BDNF、NT4/5、SIRT1、HSP70和HSP27）。对比分析了MS患者与健康对照者的这些标记物的基因表达和蛋白质水平。血液样本来自42名MS患者和48名无MS的对照对象。通过定量实时PCR技术测量特定基因的表达。样本进行双重分析，并使用2-ΔCt方法对mRNA的丰度进行量化。采用ELISA测定法测量血浆样本中特定蛋白质的浓度。研究结果揭示，BDNF基因表达下降3.5倍，其相应的血浆蛋白浓度下调1.5倍（P < 0.001）。类似趋势在NT-4（下降5倍，蛋白水平略有上升）、SIRT1（下降2倍，蛋白水平下降2倍）、HSP70（上升4倍，蛋白水平上升近2倍）和HSP27（上升4倍，蛋白水平上升2倍）中也被观察到（P < 0.001）。本研究揭示了MS患者中基因表达与蛋白质浓度之间的强烈相关性，突显了这些神经保护标记物在疾病中的相关性。