Transcription profiling by array of Ubiquitin Specific Protease 2 (Usp2) knockout mice to investigate the renal transcriptome

Renal transcriptome analysis of Usp2 knockout mice. Please note 'Zeitgeber time 12' means 12 hours after light onset in 12h: light 12h dark housing conditions. It is of importance in the present design because of the strong circadian oscillation of the interest gene. In the Characteristics [genetic modification] column Usp2-Lac0-SA-IRES-lacZ-WT Neo/Kan is the name of the targeting cassette which was inserted in the gene, in opposition to none for the WT who carry no mutation.本研究为Usp2基因敲除小鼠的肾脏转录组分析。请注意，「授时因子时间12（Zeitgeber time 12）」指的是在12小时光照、12小时黑暗的饲养环境中，光照启动后的第12小时。该参数在本实验设计中至关重要，因为目标基因存在强烈的节律振荡现象。在「[基因修饰]特征」列中，Usp2-Lac0-SA-IRES-lacZ-WT Neo/Kan为插入至目标基因的靶向载体盒名称；未携带任何突变的野生型（Wild Type, WT）样本则标注为「无」。