Characterization of NgAgo cleavage sites

NgAgo and mutants were incubated with plasmid DNA and 5'P guide sequences targeting the plasmid. Nicked/cleaved products were amplified via primer extension, which terminates at the nick site, before being used to generate dsDNA that was ligated into pGEM-T-Easy. The library of ligated products were then sequenced. Raw reads from products generated via primers hybridizing on the 5' and 3' end of the target are deposited here.