Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens

Delineating the complex network of interactions between antigen-specific T cells and antigen presenting cells (APCs) is crucial for effective precision therapies against cancer, chronic infections, and autoimmunity. However, the existing arsenal for examining antigen-specific T cell interactions is restricted to a select few antigen-T cell receptor pairs, with limited in situ utility. This lack of versatility is largely due to the disruptive effects of reagents on the immune synapse, which hinder real-time monitoring of antigen-specific interactions. To address this limitation, we have developed a novel and versatile immune monitoring strategy by adding a short cysteine-rich tag to antigenic peptides that emits fluorescence upon binding to thiol-reactive biarsenical hairpin compounds. Our findings demonstrate the specificity and durability of the novel antigen-targeting probes during dynamic immune monitoring in vitro and in vivo. This strategy opens new avenues for biological validatio..., The dataset was collected from flow cytometry and dynamic two photon imaging. The raw data are being deposited here., , # Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens

Give a brief summary of dataset contents, contextualized in experimental procedures and results.

## Description of the data and file structure

The flow cytometry and imaging data for the Figures 1-6 are deposited here as fcs, ims and pzfx files. The files are:

| Filename | File type | Figure  | Experimental Condition |
| :------------------------------------------------- | :-------- | :------ | :---------------------- |
| 6UBA70\_1.FCS | fcs | 1b | OVA |
| 6U1A28\_1.FCS | fcs | 1b | C variant |
| 6UMFLA\_2.FCS | fcs | 1b | N variant |
| 6U353A\_1.FCS | fcs | 1b | CACA |
| 6UEB09\_1.FCS ...